Translational_Unit

Part:BBa_K1202108

Designed by: mer Bilgi   Group: iGEM13_ATOMS-Turkiye   (2013-09-24)

pLsr promoter and LsrR Generator

Lsr operon is one of the existing operons in E.coli for selective production of designated proteins in different conditions. This production chain is regulated strictly by using repressors and activators. In Lsr system, LsrR is the main repressor of the promoter and can only be inhibited by the presence of phosphorylated autoinducer-2 (AI-2) quorum sensing unit. (see diagram) We want to use this part to conduct cancer detecting mechanism in bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 519
    Illegal AgeI site found at 1673
    Illegal AgeI site found at 1785
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None